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. 2016 Jun 1;7:831. doi: 10.3389/fmicb.2016.00831

Table 1.

Cq results according to DNA extraction (Ausubel and Lipp methods) with (+) or without (−) PVPP for A. aceti, A. pasteurianus, G. oxydans, and Ga. liquefaciens in (A) growth medium supplemented with 10% (v/v) ethanol, and (B) white, and (C) red wines.

(A)
Methods PVPP Ethanol medium
A. aceti A. pasteurianus G. oxydans Ga. liquefaciens
Ausubel 32.0 ± 0.0b 30.5 ± 0.0c 21.8 ± 1.8a 23.0 ± 1.7b
+ 22.7 ± 1.7a 18.3 ± 0.8b 22.4 ± 1.7a 23.9 ± 0.4b
Lipp 18.5 ± 0.6a 14.0 ± 0.5a 18.6 ± 1.7a 20.3 ± 0.6a
+ 20.4 ± 2.7a 15.4 ± 0.6a 18.9 ± 0.8a 20.2 ± 0.6a
(B)
Methods PVPP White wine
A. aceti A. pasteurianus G. oxydans Ga. liquefaciens
Ausubel 23.9 ± 1.3b 21.8 ± 0.8a 22.4 ± 0.3c 23.1 ± 1.3b
+ 23.0 ± 2.8ab 22.2 ± 0.6a 22.2 ± 1.3c 21.6 ± 0.0ab
Lipp 19.8 ± 0.1ab 19.8 ± 0.6a 16.8 ± 0.6a 21.2 ± 0.3a
+ 19.6 ± 0.8a 19.3 ± 2.3a 19.4 ± 0.7b 20.8 ± 0.4a
(C)
Methods PVPP Red wine
A. aceti A. pasteurianus G. oxydans Ga. liquefaciens
Ausubel 26.4 ± 1.9b 28.4 ± 0.5b 25.9 ± 1.7b 26.2 ± 1.1b
+ 27.8 ± 0.8b 26.9 ± 0.9b 26.3 ± 0.8b 26.1 ± 0.3b
Lipp 23.2 ± 0.5a 12.7 ± 0.7a 23.8 ± 1.0ab 21.7 ± 0.4a
+ 22.4 ± 0.4a 11.2 ± 0.3a 22.1 ± 0.6a 21.2 ± 0.0a

Values represent the Cq mean ± Standard Deviation (SD); n = 3; values followed by different letters within a column are statistically different at p < 0.05 (XLStat©).