Table 2.
Averages of E. coli K12 ER2738 found after initially adding 4 log cells/mL in ethanol growth medium and white and red wines containing A. aceti, A. pasteurianus, G. oxydans, and Ga. liquefaciens, and following DNA extraction.
| Methods | PVPP | Ethanol medium | White wine | Red wine | |||
|---|---|---|---|---|---|---|---|
| log E. coli/mL | p-values | log E. coli/mL | p-values | log E. coli/mL | p-values | ||
| Ausubel | − | 3.4 ± 0.7a | p > 0.05 | 3.6 ± 0.5a | p > 0.05 | 1.9 ± 0.5b | p < 0.05 |
| + | 3.5 ± 0.4a | p > 0.05 | 3.7 ± 0.5a | p > 0.05 | 1.8 ± 0.2b | p < 0.05 | |
| Lipp | − | 3.9 ± 0.3a | p > 0.05 | 3.6 ± 0.1a | p > 0.05 | 3.3 ± 0.2a | p > 0.05 |
| + | 3.6 ± 0.2a | p > 0.05 | 3.6 ± 0.3a | p > 0.05 | 3.7 ± 0.1a | p > 0.05 | |
The Lipp and Ausubel methods were used with (+) or without (−) PVPP during cell lysis. qPCR was done with EC23S primers.
An Anova representation with Tukey's test was applied to the E. coli enumeration results using as control modality of 4.0 log E. coli/mL, (XLStat©). Values followed by different letters within a column are statistically different at p < 0.05 (XLStat©).