Figure 4.

Clinical assessment of experimental autoimmune encephalomyelitis (EAE) mice. Clinical signs (n =8 mice/group) were monitored daily and the EAE mice were compared for 35 dpi. The intervention protocol was started after observation of the first signs of EAE induction and was continued for 2 weeks. Differences were observed between the EAE mice in different groups from 11 to 15 dpi (*P< 0.05). In order to study the interactions between riboflavin and INFβ-1a on motor disability score, GEE (generalized estimating equations) model was used. Test of Model Effect indicated that concomitant use of riboflavin and INFβ-1a has a better effect than the use of INFβ-1a alone. ST1 (sham treatment 1, EAE+ Veh1); ST2 (sham treatment 2, EAE +Veh2); T1 (treatment 1, EAE + INFβ-1a); T2 (treatment 2, EAE +riboflavin); T3 (treatment 3, EAE + INFβ-1a + riboflavin). (n= 8 for all groups)