Figure 4.

Differential phosphorylation levels of AMP-activated protein kinase (AMPK) α-Thr¹⁷² in all the neonatal rat cardiac fibroblast groups. Cells were incubated with compound C (10 µM) for 1 h in advance, then treated with AICAR and infected with CVB3 for 48 h. Phosphorylation levels of AMPK α-Thr¹⁷² were detected, and the same membrane was stripped and reprobed with GAPDH antibody. Data are given as the mean ± standard error of the mean (n=3). *P<0.05 vs. the control group; ^†P<0.05 vs. the CVB3 + AICAR group. AICAR, 5-aminoimidazole-4-carboxamide-ribonucleoside; CVB3, coxsackievirus B3; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.