Figure 1.

YAP is positively regulated by MCAM. (A and B) Representative western blotting images and quantification of western blots of YAP and MCAM in human HCC Bel-7402 cells under various treatments. The results demonstrated that MCAM regulated YAP protein expression. (C) MCAM affected YAP activity. The pUAS-Luc-TEAD-Gal4 luciferase reporter systems were transfected into human HCC Bel-7402, SMMC-7721 or Huh7 cells with or without MCAM-knockdown or overexpression. (D) MCAM regulated YAP mRNA levels. mRNA levels of YAP were measured by quantitative polymerase chain reaction in Bel-7402, SMMC-7721 or Huh7 cells with or without MCAM-knockdown or overexpression. Data are presented as the mean ± standard deviation of three independent experiments. Mock and GFP-sh groups were arbitrarily set to 100%. *P<0.05. YAP, yes-associated protein; MCAM, melanoma cell adhesion molecule; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; sh, small hairpin; GFP, green fluorescent protein; TEAD, TEA domain; Luc, luciferase; HCC, hepatocellular carcinoma; MOCK, control cells without treatment.