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. 2016 Jun 1;6:26954. doi: 10.1038/srep26954

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Copyright © 2016, Macmillan Publishers Limited

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HK-2 cells were transfected with or without MyD88 DNA followed by H/R injury. (a) Total proteins were extracted from HK-2 cells. MyD88 and p-IRAK4 protein levels were analyzed by Western Blot (one of three independent experiments). (b) Densitometric analysis of Western Blot results. (^*p < 0.0001). Results are expressed as mean ± s.d. (c) Nuclear proteins were extracted from HK-2 cells. An EMSA assay was used to detect NF-κB activity (one of three independent experiments). (d) Densitometric analysis of the NF-κB band in EMSA. (^*p < 0.05). Results are expressed as mean ± s.d.