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. 2016 Jun 1;6:26978. doi: 10.1038/srep26978

Figure 4. Impairment of autophagolysosome formation in the absence of Vps34 function.

Figure 4

(A,B) The lysosomal marker LAMP1 formed small puncta but did not co-localize either with LC3 or GFP-LC3, indicating defects in autophagolysosome formation. (C) LC3 and LAMP1 co-localized in WT C57BL/6 mice that were treated with rapamycin and leupeptin using subretinal injection, indicating autophagolysosome formation in WT but not Vps34∆rod mice. (D,E) Western blot and immunostaining showing ubiquitinated proteins accumulated in Vps34∆rod retina. (F) Co-localization of ubiquitin and LC3 indicates failure of autophagy-mediated degradation of ubiquitinated cargo in Vps34∆rod.