Figure 3.

Effect of allicin on the activity of PI3K/AKT in MC3T3-E1 cells treated with H₂O₂ (200 µM) for 6 h and 10, 30 or 100 µg/ml allicin for 24 h. (A) PI3K levels assessed using ELISA assay. (B) Western blotting of p-AKT and cleaved caspase 9. Quantification of western blots of (C) p-AKT and (D) C casp 9 were presented relative to tubulin. All of the experiments were performed in triplicate. *P<0.05, **P<0.01 or ***P<0.001, comparisons shown by brackets. ns, no significance; PI3K, phosphoinositide 3-kinase; p-AKT, phosphorylated AKT; C casp 9, cleaved caspase 9.