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. 2016 Jun 1;6:27042. doi: 10.1038/srep27042

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Copyright © 2016, Macmillan Publishers Limited

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(a) The construct harboring GFP-TaGT2L1D was transiently transformed to N. benthamiana leaves. (b) The construct harboring TaGT2L1D-YFP^C and TaGT2L1D-YFP^N were transiently co-transformed to N. benthamiana leaves. Constructs harboring YFP^C and YFP^N were used as negative control. All transformed leaves were stained with DAPI and then photographed by confocal microscopy. Bright field images show the complete epidermal cell profile in bright-field view, GFP/YFP or DAPI images show fluorescent signals in dark view, and Merge images show the overlay of three signals including bright field, GFP/YFP fluorescence and DAPI fluorescence. White arrows indicate the GFP or YFP fluorescence merged to the DAPI fluorescence. Scale bar = 50 μm.