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. 2016 Jun 1;130(14):1221–1236. doi: 10.1042/CS20160064

Figure 3. Neutrophil infiltration after tail surgery is associated with EMILIN1 cleavage.

Figure 3

(A) Representative H&E staining of cryostat tail section (left) and its boxed area magnification (right) at day 4 after surgery, showing an abundant neutrophil infiltrate. (B) Western blot analysis of recombinant EMILIN1 (E1) fragmentation in vitro after an 18-h incubation with the lymph extracted from WT and Emilin1−/− mice at day 4 (d4) or 7 (d7) after wounding. (C) Sivelestat, but not GM6001, blocked the enzymatic activity of the lymph on recombinant EMILIN1. (D) Coomassie Blue-stained gels show that recombinant EMILIN1 cleavage using NE was specifically blocked by sivelestat. Fibronectin was used as positive control for MMP-14 and the MMP inhibitor GM6001. (E) Western blot analysis of EMILIN1 degradation in normal (N) and lymphoedematous (L) tail tissue extracts. Scale bar=20 μm.