Skip to main content
. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Science. 2015 Dec 31;351(6271):391–396. doi: 10.1126/science.aad6780

Fig.4. tRF-Gly-GCC regulates MERVL-driven transcripts.

Fig.4

(A) Scatterplot shows mRNA abundance in ESCs transfected with GFP siRNA vs. LNA antisense to 5’ end of tRF-Gly-GCC.

(B) Effect of tRF-Gly-GCC inhibition on MERVL-regulated genes is isoacceptor-specific. Affymetrix data for ESCs transfected with LNA antisense oligos, relative to matched GFP controls, showing genes changing 2-fold in 2 or more samples.

(C) RNA-Seq data for ESCs transfected with GFP siRNA, or anti-tRF-Gly-GCC.

(D) Genomic context of tRF-Gly-GCC target genes, showing nearby MERVL LTRs.

(E) tRF-Gly-GCC inhibition affects MERVL targets in embryos. Averaged single embryo RNA-Seq data for control (n=28) or tRF-inhibited (n=27) 4-cell stage embryos. Among 2-fold upregulated genes, known MERVL targets(17) are indicated.

(F) Single embryo data for two MERVL targets.