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Influence of TGF-β1 on TNBC cell signal transduction pathways. (A) MDA-MB-231 cells were treated with 5 ng/ml TGF-β1 and then analyzed by western blot analysis. Smad2 protein and phosphorylation of Smad2 protein, P38 protein and phosphorylation of P38 protein band were increased in cells treated with TGF-β1. (B) Densitometric analysis of the western blot. Experiments were performed in triplicate (*P<0.05). TGF-β1, transforming growth factor-β1; TNBC, triple negative breast cancer.
