Figure 4.

FGFR2 is a target of miR-494. (A) The binding sequence of miR-494 in FGFR2. (B) The relative luciferase activity was significantly decreased in cells cotransfected with miR-494 mimic and wild-type FGFR2 3′-UTR in HeLa cell lines. miR-494 inhibited the expression of endogenous FGFR2, observed in terms of (C) messenger RNA and (D) protein levels. β-actin was used as an internal control. Data are presented as the mean ± standard deviation from three independent experiments. *P<0.05, compared with the (B) WT and (C) NC groups. FGFR2, fibroblast growth receptor 2; miR, microRNA; WT, wild-type; Mut, mutant, NC, negative control; UTR, untranslated region.