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Target genes and their primers used in this study
| Primer | Sequence (5′-3′) | Products sizes (bp) | Annealing (°C) | Ref. |
|---|---|---|---|---|
| icaA | Fw- CAATCAAGGCATTAAACAGGCTTC Rv- ACCTTTTCGTTTTCATTGTGCTAA |
509 | 62 | This study |
| fnbA | Fw- GATACAAACCCAGGTGGTGG Rv- TGTGCTTGACCATGCTCTTC |
191 | 55 | ( 20 ) |
| clfA | Fw- ATTCTGCTGTTAAAGGTGACACAT Rv- GTGTTGTAATTTGATCATCAGGCG |
657 | 62 | This study |
| bap | Fw- CCCTATATCGAAGGTGTAGAATTG Rv- GCTGTTGAAGTTAATACTGTACCTGC |
971 | 62 | (21) |
