Table 2.

Ki67 H-Scores of BT474 and MDA361 cells in 2D versus 3D culture in response to endocrine agents and trastuzumab

A (BT474): Ki67 antigen staining- expressed as % control
	C	H	T	F	H + T	H + F
3D	100	106	70	27	6	6
2D	100	33	62	25	6	2
B (MDAMB361): Ki67 antigen staining- expressed as % control
	C	H	T	F	H + T	H + F
3D	100	75	70	25	25	5
2D	100	9	64	5	9	5

H-Scoring of immunohistochemical staining (Fig. 5) were used to determine the percent of cells positive for this antigen after drug treatment. Cells cultured in 2D had less Ki67-positive cells after drug treatment than those cultured in 3D