Figure 3.

Effect of CUR analogues on TT-induced increases in androgen receptor reporter activity in CWR-22Rv1/AR cells. CWR-22Rv1/AR cells were seeded at a density of 0.1×10⁵ cells/ml of medium for 24 h. Then the medium was changed to RPMI-1640 without fetal bovine serum, and the cells were treated with vehicle (control) or TT (100 nM) alone or in combination with CUR and CUR analogues (1 µM) for 24 h. Luciferase activity and protein concentration were measured to determine androgen receptor reporter activity in the CWR-22Rv1/AR cells. Values are presented as the mean ± standard error of the mean from three separate experiments. TT, testosterone; CUR, curcumin; AN, (2E,6E)-2,6-bis(pyridin-n-methylene)cyclohexanone; BN, (2E,5E)-2,5-bis(pyridin-n-methylene)cyclopentanone; EN, (3E,5E)-3,5-bis(pyridin-n-methylene)-tetrahydropyran-4-one; FN, (3E,5E)-3,5-bis(pyridin-n-methylene)-tetrahydrothiopyran-4-one.