Figure 3.

Comparison of elution fractions of CNs and CCs obtained by Con A affinity chromatography. Lysates of stage II–III CNs and CCs were purified by Con A affinity chromatography, and elution fractions were examined by enzyme-linked lectin assay. (A) Con A, (B) RCA I, (C) MAL II and (D) AAL were used to detect mannosylation, galactosylation, sialylation and fucosylation, respectively. The central line represents the median, and the top and bottom lines represent the 75th and 25th percentiles, respectively. *P<0.05. CN (stage II–III), n=7; CC (stage II–III), n=7. CC, cancer tissue; CN, normal tissue; Con A, concanavalin A; RCA I, Ricinus communis Agglutinin I; MAL II, Maackia amurensis lectin II; AAL, Aleuria aurantia lectin.