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. 2016 Mar 24;35(11):1186–1203. doi: 10.15252/embj.201592901

Figure 5. Ribosome scanning is not required for degradation of silenced mRNAs.

Figure 5

  • A, B
    Tethering assay using the 8nt‐F‐Luc‐5BoxB‐poly(A) reporter in S2 cells. Firefly luciferase activities and mRNA levels were normalized to those of the Renilla luciferase and analyzed as described in Fig 2A and B.
  • C, D
    Tethering assay using the TISU‐R‐Luc‐6xMS2‐poly(A) reporter in human HEK293T cells. Samples were analyzed as described in Fig 2C and D.
  • E–G
    Tethering assay using the β‐globin‐6xMS2‐poly(A) reporter in human HEK293T cells treated with silvestrol or DMSO. Panel (E) shows the inhibitory effect of silvestrol on the translation of a cotransfected R‐Luc reporter. In panels (F, G), samples were analyzed as described in Fig 2C and D.
Data information: Bars represent mean values ± standard deviations from three independent experiments.Source data are available online for this figure.