Table 1.
Proteins identified by mass spectrometry after separation with PEP technology and Hexokinase Activity Analysis
| Sample number | Sample location in PEP plate | Tentative protein ID |
|---|---|---|
| Sample-1 | N19A | Plasminogen (PLG) |
| Sample-2 | N7B, N8B | Hemopexin (HPX) |
| Sample-3 | N22H, N23H, N22I | Complement Component 3 (C3) |
| Sample-4 | N17J, N18J | Fatty Acid Synthase (FASN) |
| Sample-5 | N18K, N19K | Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) |
| Sample-6 | N10M, N11M | Fatty Acid Synthase (FASN) |
| Sample-7 | N15M, N16M | Enolase 1 (ENO1) |
| Sample-8 | N1P, N2P, N3P | Glyceralde-3-phosphate Dehydrogenase |
| Sample-9 | N10P, N11P | Alpha-2-glycoprotein 1 (AZGP1) |
| Sample-10 | C3A | Fatty Acid Synthase (FASN) |
| Sample-11 | C9A, C9B | Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) |
| Sample-12 | C19D, C20D, C21D | Pyruvate Kinase (PKM) |
| Sample-13 | C19E, C20E, C21E | Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) |
| Sample-14 | C14O, C14P | Fatty Acid Synthase (FASN) |
| Sample-15 | C21P | Fatty Acid Synthase (FASN) |
*The location of each protein on the 2-D gel was defined by two parameters: the serum group and the location on the 384-plate. 1. The serum group is represented by the first letter, N represents Normal Serum, and C represents Cancer Serum. For example, N19A represents sample from Normal Serum and the PEP plate well 19A