Fig. 4.

8-OHG and 8-OHdG are comparable to G and dG in activating TLR7 in vitro. Cytokine production from various immune cells was determined by ELISA. (A) Mouse BM-pDCs from WT and Tlr7 ^−/− mice were stimulated with 100 μM G analogs with or without poly U (20 μg ml^–1). Two-tailed Student’s t-test was used to evaluate the statistical significance between polyU only and guanosine derivatives with polyU (**P < 0.01). (B) Human pDCs from seven healthy donors were analyzed in the same way as in (A). (C and D) BM-cDCs (C) and BM-MCs (D) from WT or Tlr7 ^−/− mice were analyzed as in (A). Data represent the mean ± SD of triplicate samples. All the results except (B) are representative of more than three independent experiments.