Fig. 3.

CXCR6 is required for short-term IL-17A⁺ T-cell homing to atherosclerotic aortas; 40-week-old Cxcr6 ^GFP/+ Apoe ^−/− and Cxcr6 ^GFP/GFP Apoe ^−/− splenocytes were labeled with Cell Trace Violet or Far Red fluorescent dyes, mixed in a 1:1 ratio, and adoptively transferred to 40-week-old atherosclerotic Apoe ^−/− recipients. Seventy-two hours post-transfer, the recipient spleens, PALN, and aortas were assessed for donor IL-17A⁺ T cells. (A) Representative flow cytometry plots from two pooled recipient Apoe ^−/− mice. All flow cytometry plots are gated on CD45⁺ dye⁺ donor leukocytes. Recipient Apoe ^−/− PALN isotype control staining (left), and IL-17A staining in the PALNs (middle) and aortas (right) within the gated Cxcr6 ^GFP/+ and Cxcr6 ^GFP/GFP donor populations. (B and C) Quantification of the IL-17A⁺ T-cell homing results. The percentage of IL-17A⁺ Cxcr6 ^GFP/+ and IL-17A⁺ Cxcr6 ^GFP/GFP T cells that successfully migrated to the recipient Apoe ^−/− PALNs, and aortas, normalized to the starting percentage of IL-17A⁺ T cells and IL-17A⁺ Cxcr6 ^GFP/+ T cells within the recipient organs. The mean ± SEM are shown. n = 9 independent transfer experiments. *P < 0.05, ***P < 0.001. NS, not significant.