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. 2016 Mar 13;48(5):447–453. doi: 10.1093/abbs/gmw014

Figure 1.

Figure 1.

Increases in the mitochondrial mass following knockdown of Akt protein isoforms in EJ cells (A) EJ cells expressing shAkt1, shAkt2, shAkt3, or pLKO vectors (pLKO) were lysed and subjected to western blotting using the indicated antibodies. The data shown are representative of three independent experiments. (B) The mitochondrial mass was determined by flow cytometry after staining with NAO. Relative NAO fluorescence was measured in three independent experiments, and the results are presented as mean values with standard deviations. (C) The TFAM and NRF1 mRNA levels were measured by quantitative RT-PCR. The relative TFAM and NRF1 mRNA levels obtained in two independent experiments are presented as mean values with standard deviations. *P< 0.05 compared with pLKO vector control cells according to Student's t-test. NS, not significant.