Figure 2.

Gal-1 regulates fibroblast activation CAFs and NFs were transfected with Gal-1 siRNA and Gal-1 cDNA, respectively. The depletion and expression of Gal-1 were determined by qRT-PCR (A). Relative expression levels were normalized using β-actin. (B) Western blot analysis was used to analyze protein expressions of Gal-1, α-SMA, FAP, and FN. All data were expressed as mean ± SD of three experiments. **P< 0.01 vs. NF/Con, ^##P< 0.01 vs. CAF/Con. Con, controls; OE, overexpressions; KD, knockdowns.