Figure 5.

TGF-β1 expressed by breast cancer cells promotes Gal-1 expression, fibroblast transdifferentiation, and MMP-9 secretion (A) The relative expression of Gal-1 and α-SAM was analyzed by qRT-PCR. (B) The levels of secreted MMP-9 protein in the medium were determined by human MMP-9 ELISA kit. All data were expressed as mean ± SD of three experiments. **P< 0.01, ^##P< 0.01, ^{^}P< 0.05, ^{^^}P< 0.05 vs. NF/Con treated with medium; ^aaP< 0.01, ^bbP< 0.01, ^eeP< 0.01 vs. NF/Con treated with TGF-β1; ^ccP< 0.01, ^ddP< 0.01, ^ffP< 0.01 vs. NF/Con treated with BC-CM. Con, controls; KD, knockdowns.