Skip to main content
. Author manuscript; available in PMC: 2016 Nov 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2015 Oct 6;54(48):14388–14392. doi: 10.1002/anie.201506844

Figure 1.

Figure 1

Schematic design of the DNA-lipid tether system. (A) A pair of complementary DNA-lipid tethers were incorporated into two populations of liposomes, t- and v-liposomes, respectively, at a loaded lipid:oligo molar ratio of 200:1 (100 per liposome), unless otherwise specified. The t-liposome (mole%, 85% POPC, and 15% DOPS) contains co-expressed t-SNARE (syntaxin 1/SNAP25, Cys free) with loaded lipid:protein molar ratio of 200:1 (100 per liposome), and the fluorescent v-liposome (mole%, 82% POPC, 15% DOPS, 1.5% NBD-DPPE, and 1.5% Rho-DPPE) contains v-SNARE (VAMP2, Cys free) with loaded lipid:protein molar ratio of 400:1 (50 per liposome). (B) Structure of DNA-lipid tethers. From the membrane distal end, a DNA-lipid tether hybrid molecule contains a 21 bp hybridization region (purple), a linker region containing 5 to 63 consecutive nucleotides, Thymidine (T) (orange), and a thiol-maleimide linked membrane anchor.