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. 2016 Jun 1;15:91. doi: 10.1186/s12934-016-0490-y

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Production of (R)-3-hydroxybutyrate in E. coli AF1000 a pJBGT3RX, reference cultivation. b pJBGT3RX with excess of glutamate in the medium c pJBGT3RX pBADzwf, in batch mode during ammonium depletion. Symbols refer to cell dry weigh (CDW, filled circles), glucose (Glc, crosses), glutamate (Glu, open squares), ammonium (NH₄ ⁺, closed squares), 3HB (open triangles) and acetic acid (HAc, closed triangles). The specific productivity of 3HB has been curve fitted to a 1st order polynomial. The dashed line marks the shift between batch cultivation and nitrogen depletion