Abstract
Background
In this study, our aim was to identify Candida species isolated from bloodstream infections and to determine their susceptibilities to various antifungal agents to demonstrate the local resistance profiles and to guide empirical treatment for clinicians.
Methods
Two hundred Candida isolates (95 Candida albicans, 105 non-albicans Candida strains) were included in the study. Candida species were identified by conventional, biochemical and molecular methods. Antifungal susceptibility tests for amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin and anidulafungin were performed with broth microdilution method according to the Clinical and Laboratory Standards Institute M27-A3 document.
Results
Of the 200 Candida strains, the most prevalent species were C. albicans (47.5 %), Candida glabrata (18.0 %) and Candida parapsilosis complex (14.0 %). All Candida species except for three (1.5 %) Candida kefyr strains were susceptible to amphotericin B. Only one (2.8 %) C. glabrata was resistant to fluconazole (MIC ≥ 64 μg/ml), and the others (97.2 %) exhibited dose-dependent susceptibility. All species, but C. glabrata strains, were susceptible to fluconazole. Resistance to voriconazole, posaconazole, caspofungin and anidulafungin was not detected in any strain.
Conclusion
Candida albicans were susceptible to all antifungal drugs. Three C. kefyr strains were resistant to amphotericin B. Only one C. glabrata was resistant to fluconazole. All the strains were susceptible to voriconazole, posaconazole, caspofungin and anidulafungin. In vitro antifungal susceptibility tests should be performed to select of appropriate and effective antifungal therapy, and monitor the development of resistance.
Keywords: Candida, Anti-fungal susceptibility, Microdilution method
Background
Candida species are common in nature and the flora of the human skin and mucosa and have been reported more frequently as pathogens. This is because of the risk factors such as increased use of broad-spectrum antibiotics, underlying malignant diseases, HIV/AIDS, organ transplantation, prolonged hospital stay, and exposure to invasive procedures, various opportunistic fungal infections have increased [1, 2].
Candida species can lead to a wide range of serious infections including blood stream infections (BSIs) and disseminated candidiasis. Candida species are fourth most frequent pathogens in BSIs. In spite of the advances in the diagnosis and treatment of candidiasis, the infections still have high mortality rates [3, 4].
In recent years, a gradual increase in the fungal diseases and the widespread use of empirical antifungals caused the emergence of resistant strains of fungi. Therefore, in vitro antifungal susceptibility testing requirements are increasing to select appropriate and effective antifungal therapy. The main purpose for using these tests to enable to anticipate the clinical success during the treatment of infections [5].
Antifungal susceptibility tests provide useful information to the clinicians about empirical treatments. There are some studies on the antifungal susceptibility of Candida species with broth microdilution method from Turkey [6–8], but there is no published research evaluated according to species-specific clinical breakpoints in the Clinical and Laboratory Standards Institute (CLSI) M27-S4 document. In this study, our aim was to identify Candida species isolated from BSIs and to determine the susceptibility to various antifungals including amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin, and anidulafungin to demonstrate the local resistance profiles and to guide empirical treatment for clinicians.
Methods
This study was approved by Ethical Committee of Faculty of Medicine, Selcuk University (2011, 13).
A total of 200 Candida isolates (Candida albicans: 93, Candida glabrata: 36, Candida parapsilosis complex: 28, Candida tropicalis: 24, Candida kefyr: 10, Candida lusitaniae: 7, and Candida dubliniensis: 2) were collected between 2010 and 2013 from blood cultures of hospitalized patients in various departments of the Selcuk University, Faculty of Medicine. Candida strains isolated from the clinical samples were identified by conventional methods (germ tube test, morphology on corn meal agar) and API ID 32C (bioMerieux, France) according to the manufacturer’s instructions. The isolates were stored at −70 °C in the Brain Heart Infusion broth (Oxoid, United Kingdom) with 20 % glycerol until they were studied.
After the stored isolates were subcultured, DNA was extracted using a commercial DNA isolation kit (Gentra Puregene Yeast/Bact. Kit, Qiagen, Valencia, CA, USA) according to the manufacturer’s recommendations. The primers (CR-f 5′-GCTACCACTTCAGAATCATCATC-3′ and CR-r 5′-GCACCTTCAGTCGTAGAGACG-3′) encoding hypha l wall protein 1 (HWP1) gene were selected to identify C. albicans and C. dubliniensis correctly. The PCR conditions were performed as described before [9]. PCR amplification products were electrophoresed on 1.3 % (wt/vol) agarose gel and visualized by staining with ethidium bromide (0.5 µg/mL) using a GelDoc imaging system (BioRad, Hercules, CA, USA). The isolates yielded a DNA fragment of 1000 bp were identified as C. albicans. The molecular identification of C. parapsilosis complex was performed by sequencing the internal transcribed spacer (ITS) region with ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′-TCTTTTCCTCCGCTTATTGATATG-3′) primers as previously described [10]. The amplicons were purified with commercial kit (QIAquick PCR Purification Kit, Qiagen, Valencia, CA, USA) and were analyzed by the use of an ABI 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA). The sequences were evaluated using the Sequencer 5.3 software and compared with GenBank.
Antifungal susceptibility tests were performed by broth microdilution method as described in the CLSI M27-A3 document [11]. The following antifungals were used: amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin (Sigma-Aldrich, St. Louis, MO, USA) and anidulafungin (Pfizer, New York, NY, USA). The antifungal agents and concentration ranges were between 0.015 and 16 µg/mL for amphotericin B, voriconazole and posaconazole; 0.12–64 µg/mL for fluconazole; 0.008–8 µg/mL for anidulafungin and caspofungin. The minimum inhibitory concentration (MIC) values for all agents were read following 24 h of incubation at 35 °C. The MIC values were visually determined at the lowest concentration of drug that prevents any noticeable growth for amphotericin B and a significant reduction of growth (≥50 %) for the azoles and the echinocandins compared with the drug-free growth control. Species-specific clinical breakpoints in the M27-S4 document were used for categorical evaluation [12]. Due to the lack of published breakpoints by CLSI for posaconazole, the voriconazole breakpoints were used for posaconazole. The isolates had ≤1 µg/mL MIC for AmB were accepted susceptible according to CLSI M27-S3 [13]. C. parapsilosis ATCC 22019 and C. krusei ATCC 6258 were used as quality control strains.
Results
A total of 93 C. albicans and two C. dubliniensis isolates were identified as C. albicans by HWP1 gene polymorphisms. The 28 C. parapsilosis complex isolates were sequenced, and all of the strains were identified as C. parapsilosis. None of the C. parapsilosis complex strains was identified Candida metapsilosis or Candida orthopsilosis.
Antifungal susceptibility results and MIC values as well as categories of the isolates were presented in Table 1. All Candida species except for C. kefyr were susceptible to amphotericin B. MIC values of three C. kefyr strains were 2 µg/mL for AmB. AmB had the lowest MIC90 value (0.25 µg/mL) against C. albicans and C. parapsilosis. Only one C. glabrata strain was resistant to fluconazole (MIC = ≥64 μg/mL), and the others showed dose-dependent susceptibility. The other Candida species were susceptible to fluconazole. All strains were susceptible to voriconazole, posaconazole, caspofungin and anidulafungin. Voriconazole and posaconazole had the same MIC90 value (0.06 µg/mL), but posaconazole had the lower MIC50 value (≤0.015 µg/mL) than voriconazole (0.03 µg/mL). By having MIC90 values of 0.06 µg/mL, caspofungin and anidulafungin were potentially active agents against Candida species.
Table 1.
In vitro susceptibilities of Candida species to antifungal agents determined by the broth microdilution method
| Species (number) and antifungals |
MIC range (µg/ml) |
MIC50
(µg/ml) |
MIC90
(µg/ml) |
S (%) | S-DD or I (%) |
R (%) |
|---|---|---|---|---|---|---|
| Candida spp. (200) | ||||||
| Amphotericin B | 0.06–2.0 | 0.12 | 0.5 | 100 | – | 0 |
| Fluconazole | 0.12– ≥ 64.0 | 0.25 | 1.0 | – | – | – |
| Voriconazole | ≤0.015–0.12 | 0.03 | 0.06 | – | – | – |
| Posaconazole | ≤0.015–0.12 | ≤0.015 | 0.06 | – | – | – |
| Caspofungin | ≤0.008–0.12 | 0.015 | 0.06 | – | – | – |
| Anidulafungin | ≤0.008–0.12 | 0.03 | 0.06 | – | – | – |
| C. albicans (95) | ||||||
| Amphotericin B | 0.12–1.0 | 0.12 | 0.25 | 100 | – | 0 |
| Fluconazole | 0.12–2.0 | 0.25 | 0.5 | 100 | 0 | 0 |
| Voriconazole | ≤0.015–0.06 | ≤0.015 | 0.03 | 100 | 0 | 0 |
| Posaconazole | ≤0.015–0.12 | ≤0.015 | 0.03 | 100 | 0 | 0 |
| Caspofungin | ≤0.008–0.12 | 0.015 | 0.06 | 100 | 0 | 0 |
| Anidulafungin | 0.015–0.12 | 0.015 | 0.03 | 100 | 0 | 0 |
| C. glabrata (36) | ||||||
| Amphotericin B | 0.06–1.0 | 0.12 | 0.5 | 100 | – | 0 |
| Fluconazole | 0.12– ≥ 64.0 | 1.0 | 4.0 | – | 97.2 | 2.8 |
| Voriconazole | ≤0.015–0.12 | 0.03 | 0.06 | – | – | – |
| Posaconazole | ≤0.015–0.12 | 0.06 | 0.12 | – | – | – |
| Caspofungin | ≤0.008–0.12 | 0.06 | 0.12 | 100 | 0 | 0 |
| Anidulafungin | 0.015–0.12 | 0.03 | 0.06 | 100 | 0 | 0 |
| C. parapsilosis (28) | ||||||
| Amphotericin B | 0.06–0.5 | 0.12 | 0.25 | 100 | – | 0 |
| Fluconazole | 0.12–0.5 | 0.12 | 0.5 | 100 | 0 | 0 |
| Voriconazole | ≤0.015–0.03 | ≤0.015 | 0.03 | 100 | 0 | 0 |
| Posaconazole | ≤0.015–0.03 | ≤0.015 | 0.03 | 100 | 0 | 0 |
| Caspofungin | 0.015–0.12 | 0.015 | 0.06 | 100 | 0 | 0 |
| Anidulafungin | 0.015–0.12 | 0.015 | 0.06 | 100 | 0 | 0 |
| C. tropicalis (24) | ||||||
| Amphotericin B | 0.12–0.5 | 0.25 | 0.5 | 100 | – | 0 |
| Fluconazole | 0.12–1.0 | 0.25 | 1.0 | 100 | 0 | 0 |
| Voriconazole | ≤0.015–0.06 | 0.03 | 0.06 | 100 | 0 | 0 |
| Posaconazole | ≤0.015–0.03 | ≤0.015 | 0.03 | 100 | 0 | 0 |
| Caspofungin | ≤0.008–0.06 | 0.015 | 0.06 | 100 | 0 | 0 |
| Anidulafungin | 0.015–0.06 | 0.03 | 0.06 | 100 | 0 | 0 |
| C. kefyr (10) | ||||||
| Amphotericin B | 0.12–2.0 | 0.5 | 2.0 | 70 | 0 | 30 |
| Fluconazole | 0.12–0.5 | 0.25 | 0.25 | – | – | – |
| Voriconazole | ≤0.015–0.03 | ≤0.015 | 0.03 | – | – | – |
| Posaconazole | ≤0.015–0.03 | ≤0.015 | 0.03 | – | – | – |
| Caspofungin | ≤0.008–0.03 | 0.015 | 0.03 | – | – | – |
| Anidulafungin | 0.015–0.12 | 0.03 | 0.06 | – | – | – |
| C. lusitaniae a (7) | ||||||
| Amphotericin B | 0.12–0.25 | – | – | – | ||
| Fluconazole | 0.12–1.0 | – | – | – | ||
| Voriconazole | ≤0.015–0.6 | – | – | – | ||
| Posaconazole | ≤0.015–0.6 | – | – | – | ||
| Caspofungin | ≤0.008–0.03 | – | – | – | ||
| Anidulafungin | 0.015–0.06 | – | – | – | ||
S susceptible; S-DD susceptible-dose dependent; I intermediate; R resistant
aA categorical evaluation was not performed because the number is smaller than ten
Discussion
The surveillance programs have performed invasive Candida infections and investigated the distribution of species and antifungal susceptibility. In this study, we identified Candida species isolated from BSIs and determined their susceptibilities to amphotericin B, fluconazole, voriconazole, posaconazole, caspofungin, and anidulafungin using species-specific clinical breakpoints in the M27-S4 document.
Candida albicans is responsible for about 50 % of systemic infections caused by Candida species, making it the most common infectious Candida agent [14–16]. The changes in the distribution of Candida species have been observed and the rates of non-albicans species such as C. glabrata, C. tropicalis and C. parapsilosis have been increasingly reported. C. albicans still is the most common agent in many studies although its proportion significantly decreased from 64 to 45 % in Asia–Pacific and from 68 to 50 % in Europe recently [16, 17]. Even the distribution of some species shows variation in different regions of the same country. In a study from Italy, C. parapsilosis was higher in the center and in south than the north (25.7 vs. 19.9 %). In addition, C. glabrata was higher in the south than in any other region (7.5 vs. 15.9 %) [18]. In this study, the prevalence was as follows: C. albicans, (47.5 %) C. glabrata (18.0 %), C. parapsilosis (14.0 %), C. tropicalis (12.0 %), C. kefyr (5.0 %) and C. lusitaniae (3.5 %). Previous studies reported C. albicans is the most common agent of candidemia in Turkey [6, 7, 19], except one study reported that C. parapsilosis was the most common pathogen at the rate of 55.4 % in blood cultures in İzmir [20].
In recent years, the low rates of amphotericin B resistance have been reported in the C. albicans, C. glabrata, C. parapsilosis, C. tropicalis, C. kefyr and C. krusei isolates [7, 21, 22]. However, in a study conducted by Ruan et al. [23], the resistance was extremely high against amphotericin B in C. krusei strains (95 %) and in C. glabrata strains (53 %). In our study, all strains except for C. kefyr had MICs of ≤1 µg/mL for amphotericin B. Amphotericin B has had the lowest MIC90 value (0.25 µg/mL) against C. albicans and C. parapsilosis. In previous studies from Turkey, amphotericin B resistance rates ranged from 0 to 3.8 % [6, 7, 24], which was compatible (1.5 %) with this study.
Although, fluconazole is most frequently used an agent in the treatment of systemic yeast infections, resistance rates have been reported for C. albicans (5.7–5.8 %) and for C. tropicalis (6.2–9.8 %) [25–27]. Globally, C. glabrata showed the higher resistant rates (7.7–11.9 %) than other Candida species [16, 27–29]. In Turkey, resistance rates to fluconazole is low [6, 8], but the higher resistant rate (12.8 %) in C. glabrata was reported by another study [24]. In our study, one C. glabrata strain was resistant to fluconazole, the other strains (97 %) were dose-dependent susceptible. Other Candida species were susceptible to fluconazole.
Because of a high in vitro activity, voriconazole and posaconazole are more successful choices in the treatment of fluconazole-resistant Candida species [30]. Among Candida species, alike to fluconazole, C. glabrata had the highest MIC90 values for voriconazole and posaconazole [27, 29]. However in another study the highest resistance rates to voriconazole were determined in C. tropicalis (17.6 %), following C. krusei (7.1 %) and C. albicans (4.6 %) [25]. In our study, voriconazole and posaconazole had the same MIC90 value (0.06 µg/mL), but posaconazole had the lower MIC50 value (≤0.015 µg/mL) than voriconazole (0.03 µg/mL). The highest MIC values (0.12 µg/mL) were detected in C. glabrata isolates. All Candida species were susceptible to voriconazole and posaconazole. Moreover, so far, no voriconazole resistance has been reported from Turkey [6–8].
So far, although species-dependent resistance was undetermined, resistance to echinocandins in Candida species has been reported in some publications [28, 29, 31]. Resistance to caspofungin in C. albicans (0.2–0.5 %), in C. parapsilosis (1.9 %), in C. tropicalis (0.8 %) were low rates [27, 29]. However, the higher resistance rates to caspofungin between 5.1 and 7.9 % in C. glabrata have been reported [28, 29]. Insomuch that, C. glabrata isolates were resistant to caspofungin at rate of 100 %, but susceptible to anidulafungin [21]. In this study, caspofungin and anidulafungin were potentially active against Candida species with MIC90 values 0.06 µg/mL, and no resistant strain was detected. In Turkey, caspofungin resistance was reported in 14 C. parapsilosis isolates [8].
Conclusion
Candida albicans were susceptible to all antifungal drugs. Three C. kefyr strains were resistant to amphotericin B. Only one C. glabrata was resistant to fluconazole. All the strains were susceptible to voriconazole, posaconazole, caspofungin and anidulafungin. According to our test results, there is no remarkable antifungal resistance in our hospital. Therefore, Candida isolates should be identified at the species level and MIC values should be determined even though very little resistance was detected. This study is the first investigation using species-specific clinical breakpoints in the M27-S4 document for categorical evaluation in Turkey. In vitro antifungal susceptibility tests should be performed to select of appropriate and effective antifungal therapy, and monitor the development of resistance.
Authors’ contributions
HTD and UA participated in the design of the study and in drafting the manuscript. HT and CS carried out the laboratory studies. DF carried out revising it critically for important intellectual content. All authors read and approved the final manuscript.
Acknowledgements
This study was supported by the Coordinator of Scientific Research Projects at Selcuk University (Project Number: 12401034).
A part of this study was presented as a poster presentation in the 6th Eurasia Congress of Infectious Diseases, (24–27 September 2014) in Belgrad, Serbia.
Competing interests
The authors declare that they have no competing interests.
Contributor Information
Hatice Turk Dagi, Email: haticeturkdagi@yahoo.com.
Duygu Findik, Email: dfindik@selcuk.edu.tr.
Cigdem Senkeles, Email: allstarcigdem.87@gmail.com.
Ugur Arslan, Email: drarslanugur@gmail.com.
References
- 1.Messer SA, Jones RN, Fritsche TR. International surveillance of Candida spp. And Aspergillus spp.: report from the SENTRY antimicrobial surveillance program (2003) J Clin Microbiol. 2006;44:1782–1787. doi: 10.1128/JCM.44.5.1782-1787.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Richardson MC, Lass-Flörl C. Changing epidemiology of systemic fungal infections. Clin Microbial Infect. 2008;14:5–24. doi: 10.1111/j.1469-0691.2008.01978.x. [DOI] [PubMed] [Google Scholar]
- 3.Yapar N. Epidemiology and risk factors for invasive candidiasis. Ther Clin Risk Manag. 2014;10:95–105. doi: 10.2147/TCRM.S40160. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Sievert DM, Ricks P, Edwards JR, Schneider A, Patel J, Srinivasan A, et al. Antimicrobial-resistant pathogens associated with healthcare-associated infections: summary of data reported to the national healthcare safety network at the centers for disease control and prevention, 2009–2010. Infect Control Hosp Epidemiol. 2013;34:1–14. doi: 10.1086/668770. [DOI] [PubMed] [Google Scholar]
- 5.Pfaller MA. Antifungal drug resistance: mechanisms, epidemiology and consequences for treatment. Am J Med. 2012;125:3–13. doi: 10.1016/j.amjmed.2011.11.001. [DOI] [PubMed] [Google Scholar]
- 6.Eksi F, Gayyurhan ED, Balci I. In vitro susceptibility of Candida species to four antifungal agents assessed by the reference broth microdilution method. ScientificWorldJournal. 2013;2013:236903. doi: 10.1155/2013/236903. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Metin DY, Hilmioglu-Polat S, Samlioglu P, Doganay-Oflazoglu B, Inci R, Tumbay E. Evaluation of antifungal susceptibility testing with microdilution and Etest methods of Candida blood isolates. Mycopathologia. 2011;172:187–199. doi: 10.1007/s11046-011-9413-y. [DOI] [PubMed] [Google Scholar]
- 8.Saracli MA, Gumral R, Gul HC, Gonlum A, Yildiran ST. Species distribution and in vitro susceptibility of Candida bloodstream isolates to six new and current antifungal agents in a Turkish tertiary care military hospital, recovered through 2001 and 2006. Mil Med. 2009;174:860–865. doi: 10.7205/MILMED-D-04-7808. [DOI] [PubMed] [Google Scholar]
- 9.Romeo O, Criseo G. First molecular method for discriminating between Candida africana, Candida albicans and Candida dubliniensis by using hwp1 gene. Diagn Microbiol Infect Dis. 2008;62:230–233. doi: 10.1016/j.diagmicrobio.2008.05.014. [DOI] [PubMed] [Google Scholar]
- 10.Asadzadeh M, Ahmad S, Al-Sweih N, Khan ZU. Rapid molecular differentiation and genotypic heterogeneity among Candida parapsilosis and Candida orthopsilosis strains isolated from clinical specimens in Kuwait. J Med Microbiol. 2009;58:745–752. doi: 10.1099/jmm.0.008235-0. [DOI] [PubMed] [Google Scholar]
- 11.Clinical and laboratory standards institute . Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. 3. Wayne: CLSI document M27-A3 Clinical and laboratory standards institute; 2008. [Google Scholar]
- 12.Clinical and laboratory standards institute. Reference method for broth dilution antifungal susceptibility testing of yeasts; Informational supplement. 4th ed. Wayne: CLSI document M27-S4. Clinical and laboratory standards institute; 2012.
- 13.Clinical and laboratory standards institute . Reference method for broth dilution antifungal susceptibility testing of yeasts; Informational supplement. 3. Wayne: CLSI document M27-S3 Clinical and laboratory standards institute; 2008. [Google Scholar]
- 14.Diekema DJ, Messer SA, Boyken LB. In vitro activity of seven systemically active antifungal agents against a large global collection of rare Candida species as determined by clsı broth microdilution methods. J Clin Microbiol. 2009;47:3170–3177. doi: 10.1128/JCM.00942-09. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 15.Bassetti M, Taramasso L, Nicco E, Molinari MP, Mussap M, Viscoli C. Epidemiology, species distribution, antifungal susceptibility and outcome of nosocomial candidemia in a tertiary care hospital in Italy. PLoS One. 2011;6:e24198. doi: 10.1371/journal.pone.0024198. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Pfaller MA, Diekema DJ, Gibbs DL, Newell VA, Ellis D, Tullio V, et al. Results from the ARTEMIS DISK global antifungal surveillance study, 1997 to 2007: a 10.5 year analysis of susceptibilities of Candida species to fluconazole and voriconazole as determined by CLSI standardized disk diffusion. J Clin Microbiol. 2010;48:1366–1377. doi: 10.1128/JCM.02117-09. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17.Pfaller MA, Messer SA, Woosley LN, Jones RN, Castanheira M. Echinocandin and triazole antifungal susceptibility profiles for clinical opportunistic yeast and mold isolates collected from 2010 to 2011: application of new CLSI clinical breakpoints and epidemiological cutoff values for characterization of geographic and temporal trends of antifungal resistance. J Clin Microbiol. 2013;51:2571–2581. doi: 10.1128/JCM.00308-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 18.Morace G, Borghi E, Iatta R, Amato G, Andreoni S, Brigante G, et al. Antifungal susceptibility of invasive yeast isolates in Italy: the GISIA3 study in critically ill patients. BMC Infect Dis. 2011;11:130. doi: 10.1186/1471-2334-11-130. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Aydin F, Bayramoglu G, Guler NC, Kaklikkaya N. Tosun I. Bloodstream yeast infections in a university hospital in Northeast Turkey: a 4-year survey. Med Mycol. 2011;49:316–319. doi: 10.3109/13693786.2010.512023. [DOI] [PubMed] [Google Scholar]
- 20.Baran N, Salman IS, Yurtsever SG, Ozdemir R, Gungor S, Yurtsever S, et al. Typing of Candida species isolated from blood cultures and analysis of their in vitro antifungal susceptibilities. Afr J Microbiol Res. 2013;7:4882–4885. doi: 10.5897/AJMR2013.5600. [DOI] [Google Scholar]
- 21.Taj-Aldeen SJ, Kolecka A, Boesten R, Alolaqi A, Almaslamani M, Chandra P, et al. Epidemiology of candidemia in Qatar, the Middle East: performance of MALDI-TOF MS for the identification of Candida species, species distribution, outcome, and susceptibility pattern. Infection. 2013;42:393–404. doi: 10.1007/s15010-013-0570-4. [DOI] [PubMed] [Google Scholar]
- 22.Silva AP, Miranda IM, Lisboa C, Pina-Vaz C, Rodrigues AG. Prevalence, distribution, and antifungal susceptibility profiles of Candida parapsilosis, C. orthopsilosis and C. metapsilosis in a tertiary care hospital. J Clin Microbiol. 2009;47:2392–2397. doi: 10.1128/JCM.02379-08. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 23.Ruan SY, Chu CC, Hsueh PR. In vitro susceptibilities of invasive isolates of Candida species: rapid increase in rates of fluconazole susceptible-dose dependent Candida glabrata isolates. Antimicrob Agents Chemother. 2008;52:2919–2922. doi: 10.1128/AAC.00323-08. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 24.Kiraz N, Oz Y. Species distribution and in vitro antifungal susceptibility of clinical Candida isolates from a university hospital in Turkey over a 5-year period. Med Mycol. 2011;49:126–131. doi: 10.3109/13693786.2010.503195. [DOI] [PubMed] [Google Scholar]
- 25.Fothergill AW, Sutton DA, McCarthy DI, Wiederhold NP. Impact of new antifungal breakpoints on antifungal resistance in Candida species. J Clin Microbiol. 2014;52:994–997. doi: 10.1128/JCM.03044-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 26.Zhang L, Yang HF, Liu YY, Xu XH, Ye Y, Li JB. Reduced susceptibility of Candida albicans clinical isolates to azoles and detection of mutations in the ERG11 gene. Diagn Microbiol Infect Dis. 2013;77:327–329. doi: 10.1016/j.diagmicrobio.2013.08.018. [DOI] [PubMed] [Google Scholar]
- 27.Lockhart SR, Iqbal N, Cleveland AA, Farley MM, Harrison LH, Bolden CB, et al. Species identification and antifungal susceptibility testing of Candida bloodstream isolates from population-based surveillance studies in two US cities from 2008 to 2011. J Clin Microbiol. 2012;50:3435–3442. doi: 10.1128/JCM.01283-12. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 28.Pfaller MA, Moet GJ, Messer SA, Jones RN, Castanheira M. Geographic variations in species distribution and echinocandin and azole antifungal resistance rates among Candida bloodstream infection isolates: report from the SENTRY antimicrobial surveillance program (2008–2009) J Clin Microbiol. 2011;49:396–399. doi: 10.1128/JCM.01398-10. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 29.Wisplinghoff H, Ebbers J, Geurtz L, Stefanik D, Major Y, Edmond MB, et al. Nosocomial bloodstream infections due to Candida spp. in the USA: species distribution, clinical features and antifungal susceptibilities. Int J Antimicrob Agents. 2014;43:78–81. doi: 10.1016/j.ijantimicag.2013.09.005. [DOI] [PubMed] [Google Scholar]
- 30.Rodloff C, Koch D, Schaumann R. Epidemiology and antifungal resistance in invasive candidiasis. Eur J Med Res. 2011;16:187–195. doi: 10.1186/2047-783X-16-4-187. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 31.Chen SC, Slavin MA, Sorrell TC. Echinocandin antifungal drugs in fungal infections: a comparison. Drugs. 2011;71:11–41. doi: 10.2165/11585270-000000000-00000. [DOI] [PubMed] [Google Scholar]