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. 2016 Jun 1;7:81. doi: 10.1186/s13287-016-0339-7

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© Mathan et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Immunostaining of peripheral corneal spheres reveals expression of putative stem cell and niche markers. Spheres were imaged at 60× objective magnification by confocal microscopy and labelled with antibodies (green signal) for ∆Np63α a, ABCG2 b, ABCB5 c, notch 1 d, laminin e and keratocan f. Representative image of the secondary-antibody-only control g. Blue signals represent DAPI staining of sphere cell nuclei. Scale bar = 100 μm (Colour figure online)