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. 2016 Jun 1;7:81. doi: 10.1186/s13287-016-0339-7

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© Mathan et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Immunostaining of peripheral corneal spheres stimulated by collagen I substrate reveals expression of cell proliferation and differentiation markers. Spheres were imaged at 20× objective magnification by confocal microscopy and labelled with antibodies (green signal) for keratin 3 a and vimentin b. The secondary-antibody-only control c did not show this signal. Blue signals represent DAPI staining of DNA within cell nuclei. Red signals represent EDU-incorporated cell nuclei indicative of proliferating cells. At 60× magnification, vimentin-positive migrated cells proximal to the sphere d show a different morphology compared with distal cells e and some show positivity for the EDU cell proliferation marker (red) f. Scale bar =100 μm (Colour figure online)