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. 2016 Jun 1;7:81. doi: 10.1186/s13287-016-0339-7

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© Mathan et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Implantation of peripheral corneal spheres into donor corneoscleral rims. Spheres (arrowheads) were implanted into wedge-shaped incisions made at the limbal region. Under stereomicroscopy, the corneoscleral rim with incisions (arrows) and implanted spheres can be clearly visualized a. Combined phase-contrast and fluorescence microscopy show an implanted sphere stained positively for live cells with LIVE/DEAD® stain b. This signal is confined to the sphere and not detected in the surrounding tissue. A 40× DAPI-stained 10-μm thick cross-section of frozen-stored corneoscleral rim confirmed the absence of DAPI-positive cell nuclei prior to implantation c. Through phase-contrast microscopy, the position of the spheres in the semi-transparent region of tissue is shown d