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. 2016 May 27;473(11):1629–1640. doi: 10.1042/BCJ20160187

Figure 4. ETS2 and Twist1 bind to the siah2 promoter in H. pylori-infected GCCs.

Figure 4

(A) Western blot showing H. pylori-induced ETS2 binding with the siah2 promoter (n=3). ETS2 binds to the WT EBS only but not with the EBS-Mut oligo. Input lanes show expression of ETS2 in the nuclear lysates. HDAC1 is the loading control for nuclear lysates. (B) Western blot showing H. pylori-induced Twist1 binding with the siah2 promoter (n=3). Twist1 specifically binds to the WT TBS but not to the TBS-Mut oligo. Input lanes show expression of Twist1 in the nuclear lysates. (C) ChIP analysis of ETS2 immunocomplex for siah2 EBS. (D) ChIP analysis of Twist1 immunocomplex for siah2 TBS. IgG, immunoglobulin G; M, MW marker; NS, non-specific primer, S, specific primer.