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. 2016 May 27;473(11):1629–1640. doi: 10.1042/BCJ20160187

Figure 6. Siah2 increases migration and invasion property of H. pylori-infected GCCs.

Figure 6

(A) Transwell migration assay using siControl and siSiah2-transfected AGS cells (n=3).Cells were seeded in serum-free media on to the upper chamber of Transwell. After 24 h of infection (with or without H. pylori), migrated cells at the lower surface of the membrane were stained with haematoxylin and counted. Imaging was done using an inverted microscope. Scale shown=50 μm. Bars represent number of cells migrated from three independent experiments (mean±S.E.M.). *P<0.05. (B) Results of Transwell invasion assay on the Siah2 siRNA and siControl expressed AGS cells (infected with or without H. pylori). Cells were seeded on the upper chamber of the matrigel-precoated Transwell. Cells were allowed to invade through the membrane for 24 h. Invaded cells were counted from three independent experiments. Scale shown=50 μm. Bar graph represents the average number of invaded cells (mean±S.E.M.). *P<0.05. (C) Transwell migration was performed using empty vector and Siah2-overexpressing infected and uninfected AGS cells following procedures mentioned in Figure 6A. Scale shown=50 μm. Bars represent (mean±S.E.M.). *P<0.05. (D) Cell invasiveness was measured using empty vector and Siah2-overexpressing AGS cells with or without infection as explained in Figure 6B. Scale shown=50 μm. Bar graph represents the average number of invaded cells (mean±S.E.M.), *P<0.05.