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. 2016 May 31;11:27. doi: 10.1186/s13020-016-0097-6

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© Xian et al 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — a mRNA expression levels of antioxidant and antiapoptotic genes after OGD and GUW treatment. Ct values of the genes of interest were first normalized to intrinsic β-actin of each sample, then the fold differences among each group and control was them calculated, vs OGD by one way ANOVA, post hoc Dunnett’s test (n = 4). Status of oxidative stress after OGD and GUW treatment. b Reactive oxygen species amount in between the control, OGD and GUW -treated group. The concentration of intracellular ROS was analyzed by flow cytometry. c SOD, d GPx and e Catalase activity in NGF-treated PC12 cells in different treatment groups, vs control and vs OGD by one way ANOVA, post hoc Dunnett’s test (n = 4)