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. 2016 May 31;9:20. doi: 10.1186/s13072-016-0071-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Nascent CENP-A is distributed throughout the existing CENP-A domain, with a bias toward the edges. a Quantitation of nascent (N) and total (T) CENP-A fluorescence on single chromatin fibers divided into four equal quarters. b Distribution of CENP-A loading into the outermost versus innermost quarters of the CEN chromatin domain. c The Pearson coefficient of co-localization was used to compare positioning of nascent fluorescent CENP-A pools within the CEN chromatin domain in consecutive versus alternate cell cycles. A coefficient of 1 indicates perfect co-localization. d Quantification of nascent CENP-A loading in consecutive versus alternate cycles using object-based mass-particle coincidence. A higher value indicates stronger co-localization of CENP-A fluorescence from distinct nascent protein pools