Table 1.
(a) Control | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Basal | 10 ng/mL IL-1β | 0.5 mmol/L sodium azide | ||||||||
Initial extracellular glucose (mmol/L) | Glucose consumption (pmol per cell) | Lactate production (pmol per cell) | Lactate: glucose ratio | Glucose consumption (pmol per cell) | Lactate production (pmol per cell) | Lactate: glucose ratio | Glucose consumption (pmol per cell) | Lactate production (pmol per cell) | Lactate: glucose ratio | |
5.5 | 5.3 ± 0.3 | 7.0 ± 0.6 | 1.3 ± 0.1 | 6.0 ± 0.3* | 7.1 ± 0.6 | 1.2 ± 0.1 | 6.9 ± 0.1* | 8.8 ± 0.9 | 1.3 ± 0.1 | |
11 | 4.9 ± 0.6 | 6.4 ± 1.2 | 1.3 ± 0.2 | 8.1 ± 0.9*† | 9.7 ± 1.7† | 1.2 ± 0.1 | ||||
22 | 5.5 ± 0.5 | 7.6 ± 0.8 | 1.4 ± 0.1 | 10.0 ± 0.9*† | 13.0 ± 1.6*† | 1.3 ± 0.1 | 13.2 ± 0.8*† | 17.7 ± 2.2*† | 1.4 ± 0.2 |
(b) EV and GLUT1+ | ||||||
---|---|---|---|---|---|---|
Initial extracellular glucose (mmol/L) | Glucose consumption | Lactate production | Lactate:glucose ratio | |||
EV | GLUT1+ | EV | GLUT1+ | EV | GLUT1+ | |
5.5 | 6.5 ± 0.7 | 6.0 ± 0.1 | 7.9 ± 0.4 | 6.7 ± 0.5 | 1.2 ± 0.1 | 1.1 ± 0.1 |
22 | 6.5 ± 0.4 | 6.2 ± 0.2 | 9.3 ± 1.0 | 8.6 ± 0.3 | 1.5 ± 0.1 | 1.4 ± 0.2 |
Results are expressed as the mean ± standard error of 5–28 separate experiments
EV cells with a lentiviral empty vector, GLUT1+ cells with a lentiviral vector containing human GLUT1 cDNA
*P < 0.05 vs the respective control. † P < 0.05 vs the respective value in 5.5 mmol/L glucose