Table 1.

Glucose consumption and lactate release over 24 h, and ratio between lactate production and glucose consumption in human vascular smooth muscle cells in culture

(a) Control
	Basal				10 ng/mL IL-1β			0.5 mmol/L sodium azide
Initial extracellular glucose (mmol/L)	Glucose consumption (pmol per cell)		Lactate production (pmol per cell)	Lactate: glucose ratio	Glucose consumption (pmol per cell)	Lactate production (pmol per cell)	Lactate: glucose ratio	Glucose consumption (pmol per cell)	Lactate production (pmol per cell)	Lactate: glucose ratio
5.5	5.3 ± 0.3	7.0 ± 0.6		1.3 ± 0.1	6.0 ± 0.3*	7.1 ± 0.6	1.2 ± 0.1	6.9 ± 0.1*	8.8 ± 0.9	1.3 ± 0.1
11	4.9 ± 0.6	6.4 ± 1.2		1.3 ± 0.2	8.1 ± 0.9*†	9.7 ± 1.7†	1.2 ± 0.1
22	5.5 ± 0.5	7.6 ± 0.8		1.4 ± 0.1	10.0 ± 0.9*†	13.0 ± 1.6*†	1.3 ± 0.1	13.2 ± 0.8*†	17.7 ± 2.2*†	1.4 ± 0.2

(b) EV and GLUT1+
Initial extracellular glucose (mmol/L)	Glucose consumption		Lactate production		Lactate:glucose ratio
	EV	GLUT1+	EV	GLUT1+	EV	GLUT1+
5.5	6.5 ± 0.7	6.0 ± 0.1	7.9 ± 0.4	6.7 ± 0.5	1.2 ± 0.1	1.1 ± 0.1
22	6.5 ± 0.4	6.2 ± 0.2	9.3 ± 1.0	8.6 ± 0.3	1.5 ± 0.1	1.4 ± 0.2

Results are expressed as the mean ± standard error of 5–28 separate experiments

EV cells with a lentiviral empty vector, GLUT1+ cells with a lentiviral vector containing human GLUT1 cDNA

*P < 0.05 vs the respective control. ^† P < 0.05 vs the respective value in 5.5 mmol/L glucose