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. 2015 Nov 25;310(4):F284–F293. doi: 10.1152/ajprenal.00360.2015

Fig. 1.

Fig. 1.

Renin expression in M-1 collecting duct cells is increased by desmopressin (DDAVP) treatment. A: renin and prorenin protein bands were visualized by Western blotting using 40 μg of protein extracts. The identity of renin (∼38 kDa) and prorenin (∼45 kDa) was based on the comparison with positive controls using human recombinant renin and prorenin proteins. B: dose-response curve for DDAVP (10−10, 10−8, and 10−6 M) showed prorenin and renin protein expression levels in cell lysates. C: renin mRNA expression at 3 different doses of DDAVP. D: cAMP response element-binding protein (CREB) phosphorylation at 3 different doses of DDAVP showed a significant increase in phospho-CREB at 10−8 and 10−6 M DDAVP. E: immunofluorescence (×100) using a prorenin/renin antibody showing the increased punctuated red staining (anti-mouse IgG secondary antibody Alexa Fluor 594) in response to DDAVP (10−7 M). F: dose-response curve for DDAVP showed prorenin and renin expression levels in cell culture media (×10) concentrated. G: tolvaptan, a V2 receptor antagonist, blunted the DDAVP-mediated increase in prorenin and renin protein abundances. *P < 0.05 vs. vehicle-treated group. **P < 0.01 vs. vehicle-treated group; n = 6.