Fig. 3.

Inhibition of PKA activity diminished the DDAVP-induced CREB phosphorylation and nuclear localization in M-1 cells. A: levels of the phosphorylated form of CREB normalized by total CREB after immunoblotting. B: CREB-4′,6-diamidino-2-phenylindole (DAPI) colocalization by immunofluorescence showing that CREB is undergoing nuclear destination after DDAVP treatment; however, H89 pretreatment blunted this effect as judged by immunofluorescence colocalization (red) with DAPI (blue) staining. C: percentage of CREB-DAPI colocalization in each group *P < 0.05 vs. vehicle-treated group; n = 6.