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A: integrity of the CREB-short hairpin (sh)RNA plasmids (1–4) and scrambled control (Scr) after purification assessed by agarose gels. MW, molecular weight standard. B: green fluorescent protein (GFP)-containing shRNA plasmid (green) showing transfection efficiency. C: representative Western blot showing the knockdown of CREB protein (67 ± 9%). D: CREB-shRNA prevented the DDAVP-mediated increase in prorenin and renin upregulation in M-1 cells. *P < 0.05 vs. control group (Scr shRNA).
