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. 2015 Nov 25;310(4):F284–F293. doi: 10.1152/ajprenal.00360.2015

Fig. 5.

Fig. 5.

A: DDAVP- and ANG II-independent upregulation of prorenin and renin in vitro. M-1 cells were treated with DDAVP (10−6 M), ANG II (10−7 M), or DDAVP+ANG II for 4 h. Combined treatment did not enhance prorenin and renin protein levels. B: cAMP levels in M-1 cells subjected to various treatments. DDAVP increased cAMP levels, as well as was observed by ANG II treatment alone. ANG II+DDAVP increased cAMP levels to the same extent in M-1 cells without an additive effect. AT1 receptor blockade with losartan (Los) did not affect the increases in cAMP. Calphostin C (PKC inhibitor, 10−7 M) in the presence of ANG II and DDAVP partially reduced cAMP formation. Higher dose of calphostin C blunted this effect, indicating that the stimulation of cAMP formation by DDAVP and ANG II is dependent of PKC. *P < 0.05. **P < 0.01 vs. vehicle-treated group. #P < 0.05 vs. ANG II+DDAVP and DDAVP groups.