Fig. 3.

Upregulation of Sur1 and Trpm4 protein in TLR4-activated rat microglia in vivo. a–c Double immunofluorescence labeling of microglia (P2Y12, green) and Sur1, Trpm4, or Kir6.2 (red), following intrastriatal infusion (0.5 μL/h) of normal saline (NS) or LPS (5 μg/day) for 24 h (LPS); nuclei labeled with DAPI (blue); representative high magnification (×100) images are shown; bar graphs quantification of microglial expression of Sur1, Trpm4, or Kir6.2, expressed as fold change normalized to values with NS infusion; five replicates; *p < 0.05; **p < 0.01