Fig. 1.

Expression of viral P2/P3 transgenes in multiple organs. a Schematic diagram of the P2/P3 construct controlled by the CMV promoter. BGH pA represents the bovine growth hormone polyadenylation signal. (C57BL/6 X SJL)F1 founder mice were backcrossed to either B6 or SJL mice more than eight times. b The expression of the 3D transgene region in the brain (Br), spinal cord (SC), spleen (Spl), thymus (Thy), liver (Liv), and kidney (Kid) was examined by RT-PCR. Littermates (LM) negative for the transgene resulting from the backcrossing were used as the control mice. Amplification of the GAPDH gene was compared with the viral gene expression in the samples. Representative RT-PCRs of Tg lines in the C57BL/6 and SJL backgrounds are shown. c Relative levels of transgene expression in various organs were compared using semi-quantitative real-time PCR. DNase pre-treated total RNA was used to synthesize the cDNA. The statistical significance of the differences was analyzed by Student’s t test. *P < 0.05; **P < 0.01