Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 24;9:3101–3111. doi: 10.2147/OTT.S96561

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Li et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

miR-200b directly targets to ZEB1.

Notes: (A) TargetScan software predicated that ZEB1 was a direct target gene of miR-200b. (B) The seed sequences of miR-200b in the WT and MT 3′-UTR of ZEB1 are indicated. The luciferase activity was notably decreased in osteosarcoma U2OS (C) and MG63 (D) cells cotransfected with miR-200b mimics and pMIR-ZEB1 but unaltered in U2OS and MG63 cells cotransfected with miR-200b mimics and pMIR-Mut ZEB1. Control: cells only transfected with pMIR-ZEB1 or pMIR-Mut ZEB1, respectively. NC: cells cotransfected with scramble miR and pMIR-ZEB1 or pMIR-Mut ZEB1, respectively. **P<0.01 versus NC. The error bars indicate standard deviation.

Abbreviations: miR, microRNA; WT, wild type; MT, mutant type; UTR, untranslated region; NC, negative control.