Figure 5.

miR-200b regulates ZEB1 protein expression.

Notes: (A) Real-time RT-PCR was conducted to determine the relative miR-200b level in osteosarcoma U2OS and MG63 cells transfected with miR-200b mimic or inhibitor. (B) Western blot was conducted to examine the protein expression of ZEB1 in each group. GAPDH was used as internal reference gene. U2OS and MG63 transfected with scramble miR cells were used as control. **P<0.01. The error bars indicate standard deviation. Magnification is 40×.

Abbreviations: miR, microRNA; RT-PCR, reverse transcription-polymerase chain reaction.