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. 2016 May 24;9:3101–3111. doi: 10.2147/OTT.S96561

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© 2016 Li et al. This work is published and licensed by Dove Medical Press Limited

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Knockdown of ZEB1 inhibits osteosarcoma cell proliferation.

Notes: (A) Western blot was conducted to examine the protein expression of ZEB1 in osteosarcoma U2OS, Saos2, HOS, and MG63 and normal osteoblast cell line NHOst. (B) Western blot was conducted to examine the protein expression of ZEB1 in U2OS and MG63 cells transfected with ZEB1 siRNA. (C) MTT assay was performed to determine the cell proliferation of U2OS (left) and MG63 (right) cells in each group. U2OS and MG63 cells transfected with scramble were used as control. **P<0.01 versus control. The error bars indicate standard deviation. Magnification is 40×.

Abbreviations: siRNA, small interfering RNA; h, hours; OD, optical density.