Figure 3.

c-Myc regulates the ANRIL promoter activity, depending on the E-box element.

Notes: (A) Dual luciferase assay on H1975 cells cotransfected with firefly luciferase constructs containing the ANRIL promoter and c-Myc siRNA or the control siRNA. (B) Dual luciferase assay on A549 cells cotransfected with firefly luciferase constructs containing the ANRIL promoter and pcDNA3.1-c-Myc or pcDNA3.1. (C) Schematic of the ANRIL-promoter-luciferase construct is depicted with the locations of the E-box element and the sequences of point mutation. The red star indicates the location of the putative E-box in the promoter region of ANRIL. (D) Dual luciferase assay of H1975 cells cotransfected with the ANRIL promoter reporter constructs (mutant at the E-box element) and c-Myc siRNA or siRNA NC. (E) Dual luciferase assay of A549 cells cotransfected with the ANRIL promoter reporter constructs (mutant at the E-box element) and pcDNA3.1-c-Myc or pcDNA3.1. All of the transfections were performed in triplicate. The values are presented as the mean ± standard deviation of the ratio of firefly luciferase activity to Renilla luciferase activity and are representative of at least three independent experiments. **P<0.01.

Abbreviations: NC, negative control; siRNA, small interfering RNA.