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Cued fear conditioning in our Conditioning Home Cages (CHCs) induces robust fear memory. A separate cohort of mice underwent a cued conditioning protocol identical to that used here, and were then returned to standard housing. One month later, they were returned to the CHCs and exposed to the tone. Freezing significantly increased in response to the tone compared to pre-CS freezing levels (Wilcoxon matched-pairs t-test, **p < 0.01).
