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(a) Schematic of the acoustofluidic FACS device indicating the focusing region. The expanded image indicates the laser spot, IDT region, and sorting outlets. (b) Channel cross-section illustrating the SSAW sorting mechanism. Constructive interference of surface acoustic waves originating from both IDTs results in SSAW. Upon SSAW activation, cells/particles focused at channel middle position tend to move toward the pressure node, leading them into the target outlet.
