FIGURE 5.

Expression of stabilized β-catenin in T cells prevents activation of the LAT/PLC-γ1/Ca²⁺ pathway after TCR ligation. Purified peripheral T cells from CAR Tg mice were transduced with an EV or an adenoviral vector coding for a nondegradable form of the β-catenin (β-cat). A, To measure the Ca²⁺ release after TCR stimulation, transduced cells were stained with fluo-3 and fura red dye and incubated with a biotinylated anti-CD3 Ab. Cells were kept at 37°C and analyzed by flow cytometry for the fluo-3 and fura red expression over time before and after activation of the cells with streptavidin. Ionomycin was used as a positive control. A representative of three individual experiments is shown. B, Cells were activated with anti-CD3 and anti-CD28 for 5 min, and cell lysates were blotted with specific Abs as indicated. A representative of three individual experiments is shown.