Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 15;30(10):1187–1197. doi: 10.1101/gad.278887.116

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Schibler et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 3. — Loss of H3K4 methylation results in a thick mitotic spindle. (A) Cells bearing a SET1 catalytic mutant allele (set1G951S) display a more robust mitotic spindle staining compared with wild-type cells (WT). Immunofluorescent confocal images of wild-type and set1G951S mitotic cells are shown. Tubulin (green) and DAPI (blue) stainings were used to identify cells undergoing mitosis in an asynchronous culture. Mitotic cells are outlined in white. (B) Cell cycle arrest and release experiments reveal that Pds1 is more stable in SET1 mutants (set1G951S) than in wild-type cells. Cells were arrested in G2/M and released into fresh medium. Samples were taken at the indicated time points, and whole-cell extracts and immunoblots were prepared and probed with α-Myc antibody to assess the protein levels of Myc-tagged Pds1. Immunoblots of Pgk1 served as a loading control. Cells from the indicated time points were taken for analysis by flow cytometry. Histograms of DNA content reveal the cell cycle profile of the indicated strains at specific times after cell cycle arrest and release. (C) Serial fivefold dilution assays of wild-type and mutants with the indicated genotype placed onto either control plates (YPD) or plates containing 30 µg/mL benomyl as in previous figures. (D) Immunofluorescent confocal images of wild type and mutants with the indicated genotypes. Staining and selection of cells were preformed as in A.